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Entry M0156    2.8.4.1    coenzyme-B sulfoethylthiotransferase

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Step 03

The methyl group deprotonates Tyr367E whilst disassociating from Ni(II) in factor 430. Tyr367E immediately deprotonates coenzyme B.

This reaction is irreversible.

GIF of Reaction Step M0156.stg03



Mechanisms

Proton Transfer
Heterolysis

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Intermediate Formation
Overall Product Formed
Metal De-coordination
Proton Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr367E Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Hydrogen Bond Donor
Proton Donor
Proton Relay
Gln147 Side Chain spectator Electrostatic Stabiliser
Metal Ligand

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Coenzyme B TP79000 reactant Proton Donor
Hydrogen Bond Donor
Coenzyme M COM9700 spectator Hydrogen Bond Acceptor

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
nickel F43 700 x spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-H
O-H
Ni-C
O-H
S-H
None
C
H
Ni
O
S

View similar reactions in MACiE.


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