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Overview for MACiE Entry M0152

Version history

General Information

EC Number: 2.7.8.7 (A member of the Transferases, Transferring phosphorus-containing groups, Transferases for other substituted phosphate groups)

Enzyme Name: holo-[acyl-carrier-protein] synthase

Biological Species: Bacillus subtilis (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P96618 - Holo-[acyl-carrier-protein] synthase

Representative PDB Code: 1f7l - HOLO-(ACYL CARRIER PROTEIN) SYNTHASE IN COMPLEX WITH COENZYME A AT1.5A (Resolution = 1.50 Å).

Catalytic CATH Codes:

  • 3.90.470.20 - 4'-phosphopantetheinyl transferase
  • Unassigned Domain

Display structure information

Overall Reaction:

Image of apo-[acyl-carrier protein]

Image of CoA

right arrow

Image of acyl-carrier protein

Image of adenosine 3',5'-bisphosphate

apo-[acyl-carrier protein]
C03688
CHEBI:16139
CoA
C00010
CHEBI:57287
acyl-carrier protein
C00229
CHEBI:13534
adenosine 3',5'-bisphosphate
C00054
CHEBI:37240

Overall Comment: This enzyme requires either Mg(II) or Mn(II) for activity, however the crystal structure used contains a calcium ion where the Mg(II) or Mn(II) normally binds.


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Stepwise Description of the Reaction

Step 1Magnesium activated water deprotonates the Apo-ACP, activating it.
Step 2The activated Apo-ACP attacks the CoA in a nucleophilic addition, creating a pentavalent phosphate intermediate.
Step 3The pentavalent phosphate intermediate collapses, initiating an elimination of the adenosine 3',5'-bisphosphate product, which is reprotonated from a water molecule.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 105 A Main Chain Amide
Lys 62 B Side Chain

Metal Cofactors for M0152

Type Het group Number Chain
magnesium CA 130 A Overview

References

  1. K. D. Parris et al. (2000), Structure, 8, 883-895. Crystal structures of substrate binding to Bacillus subtilis holo-(acyl carrier protein) synthase reveal a novel trimeric arrangement of molecules resulting in three active sites.
    Medline: 10997907

Homologue information for M0152 (1f7l)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
magnesium ion binding (molecular function)
cytoplasm (cellular component)
fatty acid biosynthetic process (biological process)
lipid biosynthetic process (biological process)
holo-[acyl-carrier-protein] synthase activity (molecular function)
macromolecule biosynthetic process (biological process)
transferase activity (molecular function)
metal ion binding (molecular function)
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