Overview for MACiE Entry M0150
EC Number: 188.8.131.52 (A member of the Transferases, Transferring phosphorus-containing groups, Phosphotransferases with a phosphate group as acceptor)
Enzyme Name: nucleoside-diphosphate kinase
Biological Species: Dictyostelium discoideum (Slime mould)
Catalytic Chain UniprotKB Accession Codes:
- P22887 - Nucleoside diphosphate kinase, cytosolic
Representative PDB Code: 1ndp - ADENOSINE 5'-DIPHOSPHATE BINDING AND THE ACTIVE SITE OF NUCLEOSIDEDIPHOSPHATE KINASE (Resolution = 2.20 Å).
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Stepwise Description of the Reaction
|Step 1||The gamma phosphate of ATP deprotonates His122. His122 then attacks the gamma phosphate of ATP in a nucleophilic substitution, releasing the ADP product.|
|Step 2||The nucleoside diphosphate substrate attacks the phosphate of the phosphorylated His122 in a nucleophilic substitution. The released His122 then deprotonates the triphosphate giving the nucleoside triphosphate product.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0150
- M. C. Hutter et al. (2002), Chembiochem, 3, 643-651. The mechanism of phosphorylation of natural nucleosides and anti-HIV analogues by nucleoside diphosphate kinase is independent of their sugar substituents.
- B. Schneider et al. (2001), Eur. J. Biochem., 268, 1964-1971. Mechanism of phosphoryl transfer by nucleoside diphosphate kinase pH dependence and role of the active site Lys16 and Tyr56 residues.
- S. Gallois-Montbrun et al. (2003), Mol. Pharmacol., 63, 538-546. Structural analysis of the activation of ribavirin analogs by NDP kinase: comparison with other ribavirin targets.
- Gonin P et al. (1999), Biochemistry, 38, 7265-7272. Catalytic mechanism of nucleoside diphosphate kinase investigated using nucleotide analogues, viscosity effects, and X-ray crystallography.
- Admiraal SJ et al. (1999), Biochemistry, 38, 4701-4711. Nucleophilic activation by positioning in phosphoryl transfer catalyzed by nucleoside diphosphate kinase.
Homologue information for M0150 (1ndp)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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