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Overview for MACiE Entry M0149

Version history

General Information

EC Number: 2.3.2.13 (A member of the Transferases, Acyltransferases, Aminoacyltransferases)

Enzyme Name: protein-glutamine gamma-glutamyltransferase

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

  • P00488 - Coagulation factor XIII A chain

Representative PDB Code: 1ggt - THREE-DIMENSIONAL STRUCTURE OF A TRANSGLUTAMINASE: HUMANBLOOD COAGULATION FACTOR XIII (Resolution = 2.65 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of protein glutamine

Image of protein lysine

right arrow

Image of protein N5-alkylglutamine

Image of ammonia

protein glutamine
C02583
CHEBI:32677
protein lysine
C02188
CHEBI:32568
protein N5-alkylglutamine
C03636
CHEBI:21844
ammonia
C00014
CHEBI:16134

Overall Comment: Cys314 is activated by an Asp-His diad in a manner analogous to the Ser-His-Asp diads and exists in its thiolate form in the enzyme's native state.


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Stepwise Description of the Reaction

Step 1The thiolate of Cys314 attacks the carbonyl carbon of the protein glutamine substrate. The formed oxyanion is stabilised through a H-bond with Trp279.
Step 2The oxyanion initiates the elimination of ammonia, which achieves the correct protonation state through the deprotonation of His373.
Step 3His373 deprotonates the substrate protein lysine, which attacks the carbonyl carbon of the cysteine-bound intermediate.
Step 4The oxyanion initiates the elimination of Cys314, producing the protein N5-alkylglutamine product and regenerating the enzyme's native state.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Trp 279 A Side Chain
Cys 314 A Side Chain
Main Chain Amide
His 373 A Side Chain
Asp 396 A Side Chain
Tyr 560 A Side Chain

References

  1. L. C. Pedersen et al. (1994), Protein Sci., 3, 1131-1135. Transglutaminase factor XIII uses proteinase-like catalytic triad to crosslink macromolecules.
    Medline: 7920263

Homologue information for M0149 (1ggt)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0322 galactose oxidiase
1.1.3.9
1gog 2.60.40.10
0.07690.5124Compare

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
platelet degranulation (biological process)
protein-glutamine gamma-glutamyltransferase activity (molecular function)
extracellular region (cellular component)
cytoplasm (cellular component)
blood coagulation (biological process)
transferase activity (molecular function)
peptide cross-linking (biological process)
platelet activation (biological process)
platelet alpha granule lumen (cellular component)
metal ion binding (molecular function)
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