spacer

Entry M0146    1.97.1.2    pyrogallol hydroxytransferase

Previous Step     Next Step

Step 07

The negatively charged oxygen at the 2 position of the original pyrogallol substrate initiates an elimination reaction which produces the diquinone form of 1,2,3,5-tetrahydroxybenzene (this is the molecule that was pyrogallol) and the product phloroglucinol from the molecule that was 1,2,3,5-tetrahydroxybenzene

GIF of Reaction Step M0146.stg07



Mechanisms

Unimolecular Elimination by the Conjugate Base
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Formation
Intermediate Terminated
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His144 Side Chain spectator Hydrogen Bond Acceptor
Asp174 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr404 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 07

Metal Type Metal Identity Chain Activity Function
molybdenum 4MO 902 A spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-O
O-H
The C-C bond changes from a single to double bond
The C-C bond changes from a single to double bond
The C-C bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-C bond changes from a double to single bond
The C-C bond changes from a double to single bond
The C-O bond changes from a double to single bond
C
H
O

View similar reactions in MACiE.


spacer
spacer