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Entry M0146    1.97.1.2    pyrogallol hydroxytransferase

Next Step

Step 01

His144 deprotonates the pyrogallol substrate at the C3-OH position, the activated substrate then attacks the molybdenum cofactor in a nucleophilic substitution, resulting in the loss of a water molecule from the Mo(VI) coordination sphere.

GIF of Reaction Step M0146.stg01


Comment: Molybdenum (Mo(VI)) is coordinated to four sulfur ligands from both molybdopterin guanine dinucleotides, OG from Ser175A and a hydroxyl or water molecule [1].



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Cofactor Used
Bond Formation
Metal Coordination
Bond Cleavage
Metal De-coordination
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His144 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Asp174 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Tyr404 Side Chain spectator Hydrogen Bond Acceptor

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
molybdenum 4MO 902 A spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
Mo-O
N-H
Mo-O
O-H
None
H
Mo
N
O

View similar reactions in MACiE.


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