spacer

Entry M0144    1.20.98.1    arsenite oxidase

Previous Step

Step 05

The second single electron is transferred from Mo(V) through the pterin portion of the cofactor, main chain carbonyl of Ser238, thiolate of Cys24, an iron-sulfur cluster, the peptide bond of Ser99-Ser98, His62B, a second iron-sulfur complex and His81B and finally yields the electron to a bound azurin. This regenerates the enzyme's Mo(VI) oxidation state.

It is unknown whether this reaction is reversible or not.

GIF of Reaction Step M0144.stg05



Mechanisms

Electron Transfer

Mechanism Components

Overall Reactant Used
Cofactor Regenerated
Cofactor Used
Intermediate Formation
Electron Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser238 Main Chain Carbonyl reactant One Electron Acceptor
One Electron Donor
Electron Relay
Hydrogen Bond Acceptor
Cys24 Side Chain reactant Metal Ligand
One Electron Acceptor
One Electron Donor
Electron Relay
Ser99 Main Chain Amide reactant One Electron Acceptor
One Electron Donor
Electron Relay
Hydrogen Bond Donor
Ser98 Main Chain Carbonyl reactant Hydrogen Bond Acceptor
One Electron Acceptor
One Electron Donor
Electron Relay
His62B Side Chain reactant Hydrogen Bond Donor
Metal Ligand
One Electron Acceptor
One Electron Donor
Electron Relay
His81B Side Chain reactant Metal Ligand
One Electron Acceptor
One Electron Donor
Electron Relay

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Molybdopterin guanosine dinucleotide MGD5002 reactant Metal Ligand
Hydrogen Bond Donor
One Electron Acceptor
One Electron Donor
Electron Relay

Metal Cofactors involved in Step 05

Metal Type Metal Identity Chain Activity Function
molybdenum 4MO 5004 x reactant One Electron Donor
iron FS3 5005 x reactant Electron Relay
One Electron Acceptor
One Electron Donor
iron FES 5006 x reactant Electron Relay
One Electron Acceptor
One Electron Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
None
None

View similar reactions in MACiE.


spacer
spacer