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Entry M0143    1.20.4.1    arsenate reductase

Previous Step

Step 05

The disulfide bond between Cys82 and thioredoxin is transferred to the second thiolate of thioredoxin in a nucleophilic substitution reaction, regenerating the enzyme and producing the fully oxidised thioredoxin.

GIF of Reaction Step M0143.stg05



Mechanisms

Intramolecular Nucleophilic Substitution

Mechanism Components

Bond Formation
Bond Cleavage
Enzyme-Substrate Bond Cleavage
Intermediate Collapse
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated
Cyclisation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys10A Side Chain spectator Hydrogen Bond Acceptor
Cys10A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Thr11 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly12 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asn13 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser14 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys15 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg16A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg16A Side Chain spectator Not Active
Ser17 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys82 Side Chain reactant Nucleofuge
Cys89 Side Chain spectator Hydrogen Bond Acceptor
Asp105 Side Chain spectator Not Active

Metal Cofactors involved in Step 05

Metal Type Metal Identity Chain Activity Function
potassium K 133 _ spectator Active site structural component

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
S-S
S-S
None
S

View similar reactions in MACiE.


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