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Entry M0143    1.20.4.1    arsenate reductase

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Step 03

Thiolate of Cys89 attacks the sulfur of Cys82 in a nucleophilic substitution reaction that transfers the disulfide bond to Cys89-Cys82 and releases Cys10.

GIF of Reaction Step M0143.stg03



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Formation
Bond Cleavage
Cyclisation
Decyclisation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys10A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys10A Side Chain reactant Hydrogen Bond Acceptor
Nucleofuge
Thr11 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly12 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asn13 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser14 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys15 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg16A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg16A Side Chain spectator Not Active
Ser17 Side Chain spectator Hydrogen Bond Donor
Cys82 Side Chain reactant Electrophile
Electrofuge
Cys89 Side Chain reactant Nucleophile
Asp105 Side Chain spectator Not Active

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
potassium K 133 _ spectator Active site structural component

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
S-S
S-S
None
S

View similar reactions in MACiE.


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