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Overview for MACiE Entry M0142

Version history

General Information

EC Number: 1.18.1.2 (A member of the Oxidoreductases, Acting on iron-sulfur proteins as donors, With NAD+ or NADP+ as acceptor)

Enzyme Name: ferredoxin-NADP+ reductase

Biological Species: Bos taurus (Cow)

Catalytic Chain UniprotKB Accession Codes:

  • P08165 - NADPH:adrenodoxin oxidoreductase, mitochondrial

Representative PDB Code: 1e6e - ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIALP450 SYSTEMS (Resolution = 2.3 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of NADPH

Image of adrenodoxin

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Image of proton

Image of NADP

Image of reduced adrenodoxin

NADPH
C00005
CHEBI:16474
2 adrenodoxin
C00667
CHEBI:16341
proton
C00080
CHEBI:24636
NADP
C00006
CHEBI:18009
2 reduced adrenodoxin
C00662
CHEBI:16906

View similar reactions


Stepwise Description of the Reaction

Step 1A hydride ion is transferred from the substrate NADP to the cofactor, FAD461.
Step 2A single electron is transferred from the FAD461 to one of the substrate adrenodoxin dmers via the main chains of Ile376 and Thr377 and a second adrenodoxin dimer.
Step 3FAD461 is deprotonated by a water molecule activated by Asp159. This results in the second electron transfer from FAD461 to the second adrenodoxin dimer via the main chains of Ile376 and Thr377.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 55 A Side Chain
Asp 159 A Side Chain
Ile 376 A Main Chain Amide
Main Chain Carbonyl
Thr 377 A Main Chain Amide
Main Chain Carbonyl

Organic Cofactors for M0142

Type Identity Chain
FAD FAD 461 A Overview

References

  1. G. A. Ziegler et al. (2000), Biochemistry, 39, 10986-10995. Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
    Medline: 10998235
  2. J. J. Muller et al. (2001), J. Biol. Chem., 276, 2786-2789. Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.
    Medline: 11053423
  3. D. Beilke et al. (2002), Biochemistry, 41, 7969-7978. A new electron transport mechanism in mitochondrial steroid hydroxylase systems based on structural changes upon the reduction of adrenodoxin.
    Medline: 12069587

Homologue information for M0142 (1e6e)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0255 alcohol dehydrogenase
1.1.1.1
1mg5 3.40.50.720
0.20510.0209Compare
M0092 UDP-glucose 6-dehydrogenase
1.1.1.22
1dli 3.40.50.720
0.16430.225Compare
M0021 malate dehydrogenase (oxaloacetate-decarboxylating)
1.1.1.38
1do8 3.40.50.720
0.29260.1233Compare
M0227 GDP-L-fucose synthase
1.1.1.271
1e6u 3.40.50.720
0.14810.0818Compare
M0294 succinate dehydrogenase (ubiquinone)
1.3.5.1
1yq4 3.50.50.60
3.10.20.30
0.42850.1411Compare
M0020 succinate dehydrogenase
1.3.99.1
1qjd 3.50.50.60
0.33330.1194Compare
M0110 D-amino-acid oxidase
1.4.3.3
1c0p 3.40.50.720
0.33890Compare
M0237 pteridine reductase
1.5.1.33
2c7v 3.40.50.720
0.23180.1716Compare
M0113 sarcosine oxidase
1.5.3.1
2gb0 3.50.50.60
0.16820.1999Compare
M0116 NAD(P)+ transhydrogenase (AB-specific)
1.6.1.2
1hzz 3.40.50.720
0.12120.225Compare
M0006 glutathione-disulfide reductase
1.8.1.7
2gh5 3.50.50.60
0.41070.0895Compare
M0123 adenylyl-sulfate reductase
1.8.99.2
1jnr 3.50.50.60
0.38230.1499Compare
M0131 4-hydroxybenzoate 3-monooxygenase
1.14.13.2
1doc 3.50.50.60
0.03960.1038Compare
M0090 adenosylhomocysteinase
3.3.1.1
1b3r 3.40.50.720
0.26080.3159Compare
M0226 UDP-sulfoquinovose synthase
3.13.1.1
1qrr 3.40.50.720
0.23070.2726Compare
M0228 dTDP-glucose 4,6-dehydratase
4.2.1.46
1bxk 3.40.50.720
0.18180.2571Compare
M0188 UDP-glucose 4-epimerase
5.1.3.2
1xel 3.40.50.720
0.32250.0182Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.10.20.30

View a comparison of the other reactions in MACiE with the CATH domain 3.40.50.720

View a comparison of the other reactions in MACiE with the CATH domain 3.50.50.60


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

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catalytic activity (molecular function)
ferredoxin-NADP+ reductase activity (molecular function)
binding (molecular function)
protein binding (molecular function)
mitochondrion (cellular component)
mitochondrial matrix (cellular component)
steroid biosynthetic process (biological process)
transport (biological process)
metabolic process (biological process)
cholesterol metabolic process (biological process)
electron carrier activity (molecular function)
oxidoreductase activity (molecular function)
electron transport chain (biological process)
flavin adenine dinucleotide binding (molecular function)
NADP binding (molecular function)
iron-sulfur cluster binding (molecular function)
2 iron, 2 sulfur cluster binding (molecular function)
oxidation-reduction process (biological process)
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