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Entry M0139    1.17.1.4    xanthine dehydrogenase

Previous Step

Step 04

FAD is regenerated through a hydroxide transfer from the FAD cofactor to the substrate NAD

GIF of Reaction Step M0139.stg04



Mechanisms

Aromatic Unimolecular Elimination by the conjugate base
Hydride Transfer
Aromatic Bimolecular Nucleophilic Addition

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated
Cofactor Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu1261 Side Chain spectator Hydrogen Bond Acceptor
Arg880 Side Chain spectator Not Active
Glu802 Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
MTE MTE3003 spectator Metal Ligand
FAD FAD3005 reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Hydride Donor

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
iron FES 3001 A spectator Not Active
iron FES 3002 A spectator Not Active
molybdenum MOS 3004 x spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-H
N-H
The C-C bond changes from a single to double bond
The C-N bond changes from a single to double bond
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-C bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N

View similar reactions in MACiE.


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