Overview for MACiE Entry M0138
EC Number: 18.104.22.168 (A member of the Oxidoreductases, Acting on superoxide as acceptor, Acting on superoxide as acceptor (only sub-subclass identified to date))
Enzyme Name: superoxide dismutase
Biological Species: Saccharomyces cerevisiae (Baker's yeast)
Catalytic Chain UniprotKB Accession Codes:
- P00445 - Superoxide dismutase [Cu-Zn]
Representative PDB Code: 2jcw - REDUCED BRIDGE-BROKEN YEAST CU/ZN SUPEROXIDE DISMUTASE ROOMTEMPERATURE (298K) STRUCTURE (Resolution = 1.70 Å).
Catalytic CATH Codes:
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Overall Comment: This mechanism is supported by the structural alignment of solved crystal structures and modelling of the active site .
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Stepwise Description of the Reaction
|Step 1||Superoxide donates a single electron to the Cu(II) ion, which causes the dioxygen product to dissociate and the negatively charged His63, which is no longer stabilised by the Cu(I) ion, to deprotonate a water molecule.|
|Step 2||Cu(I) donates a single electron to a second superoxide molecule, which causes it to deprotonate His63 and a bulk solvent water, through a proton relay chain made up out of water molecules.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0138
- P. J. Hart et al. (1999), Biochemistry, 38, 2167-2178. A structure-based mechanism for copper-zinc superoxide dismutase.
Homologue information for M0138 (2jcw)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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