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Entry M0133    1.14.15.1    camphor 5-monooxygenase

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Step 05

The iron-bound oxy group abstracts a hydrogen from the camphor substrate.

GIF of Reaction Step M0133.stg05


Comment: The oxyferryl intermediate (starting state) can be envisioned as the product of an intramolecular single-electron transfer reaction from the porphyrin ligand to a putative Fe(V) centre, to give Fe(IV) moiety and a radicaloid porphyrin ligand [2].



Mechanisms

Radical Formation
Hydrogen Transfer

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg186 Side Chain spectator Hydrogen Bond Donor
Asp251 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Thr252 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Cys357 Side Chain spectator Metal Ligand
Electrostatic Stabiliser
Hydrogen Bond Acceptor
Leu358 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly359 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 05

Metal Type Metal Identity Chain Activity Function
iron HEM 417 A spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-H
The Fe-O bond changes from a double to single bond
C
Fe
H
O

View similar reactions in MACiE.


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