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Entry M0133    1.14.15.1    camphor 5-monooxygenase

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Step 03

Putidaredoxin donates a second electron to the bound peroxo moiety, which initiates a proton transfer relay through Thr252, water, Asp251, Arg186 to bulk solvent.

GIF of Reaction Step M0133.stg03


Comment: There is no direct evidence for the pathway used in this first proton transfer. We have assumed that this pathway is the same as for the second proton transfer which is seen in step 4.



Mechanisms

Electron Transfer
Radical Termination
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Proton Relay
Electron Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg186 Side Chain reactant Proton Acceptor
Proton Donor
Hydrogen Bond Donor
Proton Relay
Asp251 Side Chain reactant Hydrogen Bond Donor
Hydrogen Bond Acceptor
Proton Acceptor
Proton Donor
Proton Relay
Thr252 Side Chain reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Acceptor
Proton Donor
Proton Relay
Cys357 Side Chain spectator Metal Ligand
Electrostatic Stabiliser
Hydrogen Bond Acceptor
Leu358 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly359 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
iron HEM 417 A reactant Substrate Binding
Electron Relay
One Electron Acceptor
One Electron Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
O-H
O-H
O-H
N-H
O-H
O-H
O-H
O-H
The C-O bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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