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Entry M0132    1.14.14.3    alkanal monooxygenase (FMN-linked)

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Step 03

The carbonyl carbon of the aldehyde substrate deprotonates the peroxo-intermediate, which attacks the carbonyl carbon of the aldehyde substrate in a nucleophilic addition.

GIF of Reaction Step M0132.stg03


Comment: His44 may act as a acid/base catalyst in this step.



Mechanisms

Bimolecular Nucleophilic Addition
Proton Transfer

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His44 Side Chain spectator Hydrogen Bond Acceptor
His45 Side Chain spectator Polar Non-polar interaction

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
O-H
O-H
The C-O bond changes from a double to single bond
C
H
O

View similar reactions in MACiE.


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