spacer

Entry M0132    1.14.14.3    alkanal monooxygenase (FMN-linked)

Previous Step     Next Step

Step 02

The FMN and dioxygen radical species undergo a colligation reaction to form the FMN-peroxo adduct.

GIF of Reaction Step M0132.stg02


Comment: The generation of the excited 4a-hydroxyFMNH emitter originates from the fission of the O-O bond of an intermediate along the reaction mechanism. The intermediate formed in this step can undergo a competing dark decay via the scission of the C4a-O bond. Mutations of His45 could result in structural perturbation of the luciferase flavin site, making it less rigid and/or more solvent accessible. The C4a-O bond cleavage of flavin 4a-hydroperoxide has been shown to be effectively retarded in hydrophobic media [5].



Mechanisms

Colligation
Radical Termination

Mechanism Components

Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His44 Side Chain spectator Not Active
His45 Side Chain spectator Electrostatic Stabiliser
Polar Non-polar interaction

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
None
None
C
O

View similar reactions in MACiE.


spacer
spacer