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Entry M0131    1.14.13.2    4-hydroxybenzoate 3-monooxygenase

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Step 03

FAD undergoes a double bond rearrangement that results in the single electron transfer from FAD to dioxygen.

GIF of Reaction Step M0131.stg03



Mechanisms

Electron Transfer
Radical Formation

Mechanism Components

Overall Reactant Used
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His72 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Tyr201 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Tyr385 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Pro293 Main Chain Carbonyl spectator Not Active
Lys297 Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD395 reactant One Electron Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
The O-O bond changes from a double to single bond
C
N
O

View similar reactions in MACiE.


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