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Entry M0130    1.14.12.12    naphthalene 1,2-dioxygenase

Next Step

Step 01

NAD initiates a hydride transfer to the FAD cofactor.

GIF of Reaction Step M0130.stg01



Mechanisms

Aromatic Unimolecular Elimination by the conjugate base
Aromatic Bimolecular Nucleophilic Addition
Hydride Transfer

Mechanism Components

Overall Reactant Used
Cofactor Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His104 Side Chain spectator Metal Ligand
Hydrogen Bond Donor
Asp205E Side Chain spectator Hydrogen Bond Acceptor
His208E Side Chain spectator Metal Ligand
Hydrogen Bond Donor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD reactant Hydride Acceptor

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
iron FES 451 A spectator Not Present
iron FE 452 A spectator Not Present
iron FE(not in PDB) 1 x spectator Not Active
iron FE(not in PDB) 2 x spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-H
The C-C bond changes from a single to double bond
The C-C bond changes from a single to double bond
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-N bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N

View similar reactions in MACiE.


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