Entry M0128 18.104.22.168 photinus-luciferin 4-monooxygenase
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Water deprotonates the luciferin intermediate at the C4 atom resulting in a carbanionic intermediate
Rate Determining Step
Comment: This step occurs in the bAcs Conformation. To oxidise luciferin, luciferase must abstract the C4 proton producing a carbanion that undergoes electrophilic attack by molecular oxygen. This process is greatly facilitated by the activating effect of AMP which increases the acidity of C4 and is a good leaving group, allowing the formation of the dioxetanone ring. The abstraction of the proton from C4 is the rate-limiting step of the oxygenase reaction . Due to lack of evidence suggesting an enzymatic base we have assumed that the base is water.
Amino acids involved in the reaction step.
Metal Cofactors involved in Step 02
||MG(not in PDB) 1
||Bonds Changed in Order
||Atom Types Involved
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