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Entry M0128    1.13.12.7    photinus-luciferin 4-monooxygenase

Next Step

Step 01

The carboxylate oxygen of photinus luciferin attacks the alpha-phosphate of ATP in a nucleophilic substitution reaction, producing the pyrophosphate product.

GIF of Reaction Step M0128.stg01


Comment: This step occurs in the PheA conformation.



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys529 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Thr343A Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Thr343A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Activator
Substrate Binding
Electrostatic Stabiliser
Increase Electrophilicity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
P-O
None
O
P

View similar reactions in MACiE.


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