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Entry M0127    1.12.7.2    ferredoxin hydrogenase

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Step 03

The bridging hydride deprotonates the thiol, reforming the bound thiolate and a hydrogen molecule bound to one of the Fe(II) centres in a bidentate fashion.

GIF of Reaction Step M0127.stg03


Comment: The bond bridging the hydride ion and the two Fe(II) ions is a two-electron-three-centre (2e-3c) bond.



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Metal Coordination
Bond Cleavage
Metal De-coordination
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu156 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Glu159 Side Chain spectator Hydrogen Bond Acceptor
Cys178 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Ser198 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Lys237 Side Chain spectator Hydrogen Bond Donor
Glu240 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Glu245 Side Chain spectator Hydrogen Bond Acceptor

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
iron FE2 426 L spectator Substrate Binding
iron FE2 427 L spectator Substrate Binding
iron SF4 422 L spectator Unknown Function
iron SF4 423 L spectator Not Active
iron SF4 424 L spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
Fe-H
H-H
Fe-H
S-H
None
Fe
H
S

View similar reactions in MACiE.


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