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Entry M0126    1.12.2.1    cytochrome-c3 hydrogenase

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Step 02

Cys530 acts as a general base, deprotonating the hydrogen molecules, which forms a hydride ion that bridges the two metal centres.

GIF of Reaction Step M0126.stg02


Comment: There are numerous candidates for the general base and no experimental evidence that definitively defines the actual base. We have chosen to show Cys530 as the general base as suggested by Jayapal et al. [2].



Mechanisms

Coordination
Proton Transfer

Mechanism Components

Bond Cleavage
Cofactor Used
Bond Formation
Metal De-coordination
Metal Coordination
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg463B Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser486B Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys530B Side Chain reactant Metal Ligand
Hydrogen Bond Acceptor
Proton Acceptor
Cys65B Side Chain spectator Metal Ligand
Activator
Cys533B Side Chain spectator Metal Ligand
Activator
Electrostatic Stabiliser
Cys68B Side Chain spectator Metal Ligand
Activator
Electrostatic Stabiliser
His72B Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Glu18B Side Chain spectator Not Active

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
iron FCO 537 L spectator
iron F3S 266 S spectator
iron SF4 265 S spectator
nickel NI 538 L spectator
iron SF4 267 S spectator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
Fe-H
S-H
H-H
Ni-H
None
Fe
H
Ni
S

View similar reactions in MACiE.


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