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Entry M0122    1.8.4.11    protein-methionine-S-oxide reductase

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Step 06

Cys227 deprotonates the second thiol of thioredoxin, which initiates a nucleophilic attack on the sulfur of the thioredoxin that is covalently attached to Cys227 in a substitution reaction, eliminating Cys227.

GIF of Reaction Step M0122.stg06



Mechanisms

Intramolecular Nucleophilic Substitution
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Overall Product Formed
Enzyme-Substrate Bond Cleavage
Cyclisation
Intermediate Terminated
Intermediate Collapse

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr155 Side Chain spectator Not Active
Glu115 Side Chain spectator Not Active
Tyr103 Side Chain spectator Not Active
Cys72 Side Chain spectator Not Active
Cys218 Side Chain spectator Hydrogen Bond Donor
Cys227 Side Chain reactant Nucleofuge
Hydrogen Bond Acceptor
Proton Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
S-H
S-S
S-H
S-S
None
H
S

View similar reactions in MACiE.


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