Entry M0122 18.104.22.168 protein-methionine-S-oxide reductase
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The hydroxyl group deprotonates Cys218, which initiates a nucleophilic attack on Cys72 in a substitution reaction, eliminating water.
Comment: Collapse of the intermediate is facilitated by the concomitant proton transfer from either solvent, Cys218 or other active site donor . Here we show Cys218 as the proton donor. The formation of a disulfide bond between Cys72 and Cys218 is supported by the analysis of double mutants by mass spectrometry and by the inability of dithiothreitol to rescue the lower activity of the Cys218 variants .
Amino acids involved in the reaction step.
||Bonds Changed in Order
||Atom Types Involved
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