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Entry M0117    1.6.2.4    NADPH-hemoprotein reductase

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Step 02

Water deprotonates the N1, initiating a single electron transfer from FAD to FMN, with concomitant deprotonation of water at the FMN N5.

GIF of Reaction Step M0117.stg02


Comment: We assume a oxonium acts as a proton donor in this step.



Mechanisms

Proton Transfer
Electron Transfer
Radical Formation

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Cofactor Used
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser457 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Asp675 Side Chain spectator Hydrogen Bond Acceptor
Cys630 Side Chain spectator Hydrogen Bond Donor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD750 reactant Hydrogen Bond Donor
Proton Donor
One Electron Donor
FMN FMN751 reactant Hydrogen Bond Acceptor
Proton Acceptor
One Electron Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
N-H
O-H
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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