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Entry M0117    1.6.2.4    NADPH-hemoprotein reductase

Next Step

Step 01

NADP eliminates a hydride ion, which adds to FAD, with concomitant deprotonation of water at the FAD N1.

GIF of Reaction Step M0117.stg01


Comment: Cys630 is partially responsible for the proper orientation of the nicotinamide ring by making a van der Waals contact with the C4 atom and promoting hydride transfer by stabilising the transient carbocation formed upon release of the hydride ion. The nicotinamide ring of NADP+ is displaced by Trp677.



Mechanisms

Aromatic Unimolecular Elimination by the conjugate base
Hydride Transfer
Aromatic Bimolecular Nucleophilic Addition
Proton Transfer

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Cofactor Used
Overall Product Formed
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser457 Side Chain spectator Hydrogen Bond Acceptor
Asp675 Side Chain spectator Hydrogen Bond Acceptor
Cys630 Side Chain spectator van der Waals interaction
Steric Role
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
N-H
C-H
O-H
The C-C bond changes from a single to double bond
The C-C bond changes from a single to double bond
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-N bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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