Overview for MACiE Entry M0117
EC Number: 188.8.131.52 (A member of the Oxidoreductases, Acting on NADH or NADPH, With a heme protein as acceptor)
Enzyme Name: NADPH-hemoprotein reductase
Biological Species: Rattus norvegicus (Rat)
Catalytic Chain UniprotKB Accession Codes:
- P00388 - NADPH--cytochrome P450 reductase
Representative PDB Code: 1amo - THREE-DIMENSIONAL STRUCTURE OF NADPH-CYTOCHROME P450REDUCTASE: PROTOTYPE FOR FMN-AND FAD-CONTAINING ENZYMES (Resolution = 2.60 Å).
Catalytic CATH Codes:
- 184.108.40.206 - Translation factors
- 220.127.116.11 - Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module
"Other" CATH Codes:
Display structure information
It is unknown whether this reaction is reversible or not.
Overall Comment: This enzyme can catalyse a two-electron reduction of one hemoprotein or a one-electron reduction of two hemoproteins.
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Stepwise Description of the Reaction
|Step 1||NADP eliminates a hydride ion, which adds to FAD, with concomitant deprotonation of water at the FAD N1.|
|Step 2||Water deprotonates the N1, initiating a single electron transfer from FAD to FMN, with concomitant deprotonation of water at the FMN N5.|
|Step 3||Water deprotonates Cys630, which deprotonates Ser457 which deprotonates FAD facilitating the second single electron transfer from FAD to FMN.|
|Step 4||FMN transfers a single electron to the hemoprotein.|
|Step 5||Water deprotonates the FMN, facilitating the transfer of the second single electron to the hemoprotein.|
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Catalytic Residues Involved
||Location of Function
Organic Cofactors for M0117
- P. A. Hubbard et al. (2001), J. Biol. Chem., 276, 29163-29170. NADPH-cytochrome P450 oxidoreductase. Structural basis for hydride and electron transfer.
Homologue information for M0117 (1amo)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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