spacer

Entry M0115    1.5.99.12    cytokinin dehydrogenase

Next Step

Step 01

The nitrogen of trimethylamine initiates the elimination of a hydride ion, which is added to FAD.

GIF of Reaction Step M0115.stg01


Comment: This could be a two step reaction involving a short-lived radical species.



Mechanisms

Unimolecular Elimination by the Conjugate Base
Hydride Transfer
Aromatic Bimolecular Nucleophilic Addition

Mechanism Components

Overall Reactant Used
Cofactor Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His105 Side Chain reactant Covalently Attached
Activator
Asp169 Side Chain spectator Hydrogen Bond Acceptor
Glu288 Side Chain spectator Hydrogen Bond Donor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD1535 reactant Hydrogen Bond Acceptor
Hydride Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-H
The C-C bond changes from a single to double bond
The C-N bond changes from a single to double bond
The C-N bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N

View similar reactions in MACiE.


spacer
spacer