Overview for MACiE Entry M0115
EC Number: 18.104.22.168 (A member of the Oxidoreductases, Acting on the CH-NH group of donors, With other acceptors)
Enzyme Name: cytokinin dehydrogenase
Biological Species: Zea mays (Maize)
Catalytic Chain UniprotKB Accession Codes:
- Q9T0N8 - Cytokinin dehydrogenase 1
Representative PDB Code: 1w1o - NATIVE CYTOKININ DEHYDROGENASE (Resolution = 1.7 Å).
Catalytic CATH Codes:
Display structure information
It is unknown whether this reaction is reversible or not.
View similar reactions
Stepwise Description of the Reaction
|Step 1||The nitrogen of trimethylamine initiates the elimination of a hydride ion, which is added to FAD.|
|Step 2||The first single electron transfer from FAD to quinone. The semi-quinone undergoes double bond rearrangement that results in the deprotonation of water.|
|Step 3||The second single electron transfer from FAD to quinone which is facilitated by the abstraction of a proton from the FMN by water. The quinol deprotonates another water molecule.|
|Step 4||The product of the enzyme undergoes spontaneous hydrolysis outside of the active site to produce adenine and 3-methylbut-2-enal.|
View similar reactions (composite manual annotation)
Catalytic Residues Involved
||Location of Function
Organic Cofactors for M0115
- E. Malito et al. (2004), J. Mol. Biol., 341, 1237-1249. Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis.
- P. Galuszka et al. (2005), Plant Cell Physiol., 46, 716-728. Tissue localization of cytokinin dehydrogenase in maize: possible involvement of quinone species generated from plant phenolics by other enzymatic systems in the catalytic reaction.
- J. Frebortova et al. (2004), Biochem. J., 380, 121-130. Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors.
Homologue information for M0115 (1w1o)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
Links to this entry in other databases