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Overview for MACiE Entry M0115

Version history

General Information

EC Number: 1.5.99.12 (A member of the Oxidoreductases, Acting on the CH-NH group of donors, With other acceptors)

Enzyme Name: cytokinin dehydrogenase

Biological Species: Zea mays (Maize)

Catalytic Chain UniprotKB Accession Codes:

  • Q9T0N8 - Cytokinin dehydrogenase 1

Representative PDB Code: 1w1o - NATIVE CYTOKININ DEHYDROGENASE (Resolution = 1.7 Å).

Catalytic CATH Codes:

  • Unassigned Domain

Display structure information

Overall Reaction:

Image of N6-(delta2-Isopentenyl)-adenine

Image of quinone

Image of water

right arrow

Image of adenine

Image of hydroquinone

Image of 3-methylbut-2-enal

N6-(delta2-Isopentenyl)-adenine
C04083
CHEBI:17660
quinone
C00472
CHEBI:36141
water
C00001
CHEBI:15377
adenine
C00147
CHEBI:16708
hydroquinone
C00530
CHEBI:17594
3-methylbut-2-enal
C07330
CHEBI:15825

It is unknown whether this reaction is reversible or not.


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Stepwise Description of the Reaction

Step 1The nitrogen of trimethylamine initiates the elimination of a hydride ion, which is added to FAD.
Step 2The first single electron transfer from FAD to quinone. The semi-quinone undergoes double bond rearrangement that results in the deprotonation of water.
Step 3The second single electron transfer from FAD to quinone which is facilitated by the abstraction of a proton from the FMN by water. The quinol deprotonates another water molecule.
Step 4The product of the enzyme undergoes spontaneous hydrolysis outside of the active site to produce adenine and 3-methylbut-2-enal.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 105 A Side Chain
Asp 169 A Side Chain
Glu 288 A Side Chain

Organic Cofactors for M0115

Type Identity Chain
FAD FAD 1535 A Overview

References

  1. E. Malito et al. (2004), J. Mol. Biol., 341, 1237-1249. Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis.
    Medline: 15321719
  2. P. Galuszka et al. (2005), Plant Cell Physiol., 46, 716-728. Tissue localization of cytokinin dehydrogenase in maize: possible involvement of quinone species generated from plant phenolics by other enzymatic systems in the catalytic reaction.
    Medline: 15746157
  3. J. Frebortova et al. (2004), Biochem. J., 380, 121-130. Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors.
    Medline: 14965342

Homologue information for M0115 (1w1o)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
catalytic activity (molecular function)
extracellular region (cellular component)
extracellular space (cellular component)
UDP-N-acetylmuramate dehydrogenase activity (molecular function)
cytokinin metabolic process (biological process)
oxidoreductase activity (molecular function)
oxidoreductase activity, acting on CH-OH group of donors (molecular function)
cytokinin dehydrogenase activity (molecular function)
flavin adenine dinucleotide binding (molecular function)
oxidation-reduction process (biological process)
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