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Overview for MACiE Entry M0114

Version history

General Information

EC Number: 1.5.8.2 (A member of the Oxidoreductases, Acting on the CH-NH group of donors, With a flavin as acceptor)

Enzyme Name: trimethylamine dehydrogenase

Biological Species: Methylophilus methylotrophus w3a1 (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P16099 - Trimethylamine dehydrogenase

Representative PDB Code: 2tmd - CORRELATION OF X-RAY DEDUCED AND EXPERIMENTAL AMINO ACID SEQUENCES OF TRIMETHYLAMINE DEHYDROGENASE (Resolution = 2.40 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of electron-transferring flavoprotein

Image of trimethylamine

Image of water

right arrow

Image of proton

Image of reduced electron-transferring flavoprotein

Image of dimethylamine

Image of formaldehyde

electron-transferring flavoprotein
C04253
CHEBI:5086
trimethylamine
C00565
CHEBI:18139
water
C00001
CHEBI:15377
2 proton
C00080
CHEBI:24636
reduced electron-transferring flavoprotein
C04570
dimethylamine
C00543
CHEBI:17170
formaldehyde
C00067
CHEBI:16842

It is unknown whether this reaction is reversible or not.


View similar reactions


Stepwise Description of the Reaction

Step 1The nitrogen of trimethylamine initiates the elimination of a hydride ion, which is added to FMN.
Step 2The first single electron transfer from FMN to ETF, which passes through a single iron-sulfur cluster.
Step 3The second single electron transfer from FMN to ETF, which passes through a single iron-sulfur cluster. The transfer is facilitated by the abstraction of a proton from the FMN by water.
Step 4The product of the enzyme undergoes spontaneous hydrolysis outside of the active site to produce formaldehyde and dimethylamine.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Tyr 169 A Side Chain
His 172 A Side Chain
Cys 30 A Side Chain

Organic Cofactors for M0114

Type Identity Chain
FMN FMN 802 A Overview

Metal Cofactors for M0114

Type Het group Number Chain
iron FS4 801 A Overview

References

  1. J. Basran et al. (2001), J. Biol. Chem., 276, 24581-24587. Deuterium isotope effects during carbon-hydrogen bond cleavage by trimethylamine dehydrogenase. Implications for mechanism and vibrationally assisted hydrogen tunneling in wild-type and mutant enzymes.
    Medline: 11304539
  2. M. J. Sutcliffe et al. (2002), Eur. J. Biochem., 269, 3096-3102. A new conceptual framework for enzyme catalysis. Hydrogen tunnelling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes.
    Medline: 12084049

Homologue information for M0114 (2tmd)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0102 L-lactate dehydrogenase (cytochrome)
1.1.2.3
1fcb 3.20.20.70
0.55260.45Compare
M0108 2,4-dienoyl-CoA reductase (NADPH)
1.3.1.34
1ps9 3.20.20.70
3.40.50.720
3.50.50.60
0.380.3219Compare
M0109 dihydroorotate oxidase
1.3.3.1
1d3g 3.20.20.70
0.57140Compare
M0111 glutamate synthase (ferredoxin)
1.4.7.1
1ofd 3.20.20.70
0.28660.0692Compare
M0319 NADPH dehydrogenase
1.6.99.1
1oya 3.20.20.70
0.57890.225Compare
M0148 transaldolase
2.2.1.2
1onr 3.20.20.70
0.16420.0159Compare
M0008 nicotinate-nucleotide diphosphorylase (carboxylating)
2.4.2.19
1qpr 3.20.20.70
0.17070Compare
M0243 pyridoxine 5'-phosphate synthase
2.6.99.2
1ho1 3.20.20.70
0.19060.1420Compare
M0050 orotidine-5'-phosphate decarboxylase
4.1.1.23
1dbt 3.20.20.70
0.10340.0158Compare
M0252 indole-3-glycerol-phosphate synthase
4.1.1.48
1igs 3.20.20.70
0.32070Compare
M0236 3-dehydro-L-gulonate-6-phosphate decarboxylase
4.1.1.85
1q6l 3.20.20.70
0.33330.1499Compare
M0052 fructose-bisphosphate aldolase (Class II)
4.1.2.13
1b57 3.20.20.70
0.280Compare
M0222 fructose-bisphosphate aldolase (Class I)
4.1.2.13
2qut 3.20.20.70
0.13670.0830Compare
M0054 3-dehydroquinate dehydratase (type I)
4.2.1.10
1qfe 3.20.20.70
0.23570.18Compare
M0230 porphobilinogen synthase
4.2.1.24
1gzg 3.20.20.70
0.22840.0114Compare
M0267 dihydrodipicolinate synthase
4.2.1.52
1dhp 3.20.20.70
0.36360.1967Compare
M0270 ribulose-phosphate 3-epimerase
5.1.3.1
1h1z 3.20.20.70
0.29030.0155Compare
M0324 triosephosphate isomerase
5.3.1.1
1tph 3.20.20.70
0.31570.1499Compare
M0223 pyruvate carboxylase
6.4.1.1
2qf7 3.20.20.70
0.23450.0094Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.20.20.70

View a comparison of the other reactions in MACiE with the CATH domain 3.40.50.720

View a comparison of the other reactions in MACiE with the CATH domain 3.50.50.60


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
metabolic process (biological process)
FMN binding (molecular function)
oxidoreductase activity (molecular function)
metal ion binding (molecular function)
trimethylamine dehydrogenase activity (molecular function)
iron-sulfur cluster binding (molecular function)
4 iron, 4 sulfur cluster binding (molecular function)
oxidation-reduction process (biological process)
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