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Entry M0112    1.5.1.3    dihydrofolate reductase

Next Step

Step 01

The dihydrofolate substrate undergoes a tautomerisation reaction, resulting in the deprotonation of water.

GIF of Reaction Step M0112.stg01



Mechanisms

Proton Transfer
Assisted Other Tautomerisation

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Thr116 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp26 Side Chain spectator Hydrogen Bond Acceptor
Trp21 Side Chain spectator Hydrogen Bond Donor
Activator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
The C-N bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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