Overview for MACiE Entry M0112
EC Number: 22.214.171.124 (A member of the Oxidoreductases, Acting on the CH-NH group of donors, With NAD+ or NADP+ as acceptor)
Enzyme Name: dihydrofolate reductase
Biological Species: Lactobacillus casei (Bacteria)
Catalytic Chain UniprotKB Accession Codes:
- P00381 - Dihydrofolate reductase
Representative PDB Code: 3dfr - CRYSTAL STRUCTURES OF ESCHERICHIA COLI AND LACTOBACILLUSCASEI DIHYDROFOLATE REDUCTASE REFINED AT 1.7 ANGSTROMSRESOLUTION. I. GENERAL FEATURES AND BINDING OF METHOTREXATE (Resolution = 1.70 Å).
Catalytic CATH Codes:
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Stepwise Description of the Reaction
|Step 1||The dihydrofolate substrate undergoes a tautomerisation reaction, resulting in the deprotonation of water.|
|Step 2||NADP eliminates a hydride ion, which adds to the C6 of the pterin ring, the N5 of the pterin ring deprotonates water, which deprotonates a second water, deprotonating the hydroxyl, which regenerates the correct tautomeric form of the tetrahydrofolate product.|
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Catalytic Residues Involved
||Location of Function
- M. G. Casarotto et al. (1999), Biochemistry, 38, 8038-8044. Direct measurement of the pKa of aspartic acid 26 in Lactobacillus casei dihydrofolate reductase: implications for the catalytic mechanism.
- P. Shrimpton et al. (2002), Protein Sci., 11, 1442-1451. Role of water in the catalytic cycle of E. coli dihydrofolate reductase.
Homologue information for M0112 (3dfr)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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