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Entry M0111    1.4.7.1    glutamate synthase (ferredoxin)

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Step 06

The amine initiates an elimination of the bound hydroxide as water, which deprotonates Lys972.

GIF of Reaction Step M0111.stg06


Comment: This reaction (final step of Schiff base formation) occurs in the FMN-binding domain (CATH code 3.20.20.70). We assume that Lys972 is acting as the acid in this step as it is hydrogen bonded to the hydroxyl leaving group.



Mechanisms

Unimolecular Elimination by the Conjugate Base
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Formation
Dehydration
Schiff Base Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Gln969 Side Chain spectator Hydrogen Bond Acceptor
Gln978 Main Chain Amide spectator Hydrogen Bond Donor
Activator
Lys972 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Glu903 Side Chain spectator Hydrogen Bond Acceptor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FMN FMN2508 spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
iron F3S 2509 A spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-O
N-H
The C-N bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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