spacer

Entry M0111    1.4.7.1    glutamate synthase (ferredoxin)

Previous Step     Next Step

Step 04

The oxyanion initiates an elimination that cleaves the C-S bond, the thiolate of Cys1 deprotonates water, which deprotonates the N-terminus of Cys1

GIF of Reaction Step M0111.stg04


Comment: This reaction occurs in the amidotransferase domain represented by CATH code 3.60.20.10.



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Overall Product Formed
Enzyme Regenerated
Enzyme-Substrate Bond Cleavage
Proton Relay
Intermediate Terminated
Intermediate Collapse

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asn227 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly228 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys1A Side Chain reactant Nucleofuge
Hydrogen Bond Acceptor
Proton Acceptor
Cys1A Main Chain N Terminus reactant Hydrogen Bond Donor
Proton Donor
Arg31 Main Chain Carbonyl spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Phe207 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
iron F3S 2509 A spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
S-H
C-S
N-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O
S

View similar reactions in MACiE.


spacer
spacer