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Entry M0111    1.4.7.1    glutamate synthase (ferredoxin)

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Step 03

The N-terminus of Cys1 deprotonates water, which deprotonates another water that initiates a nucleophilic attack on the carbonyl carbon of the covalently bound intermediate in an addition reaction.

GIF of Reaction Step M0111.stg03


Comment: This reaction occurs in the amidotransferase domain represented by CATH code 3.60.20.10.



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Addition

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Proton Relay
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asn227 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly228 Main Chain Amide spectator Hydrogen Bond Donor
Cys1A Main Chain N Terminus reactant Hydrogen Bond Acceptor
Proton Acceptor
Cys1A Side Chain reactant Covalently Attached
Hydrogen Bond Acceptor
Arg31 Main Chain Carbonyl spectator Hydrogen Bond Acceptor
Activator
Phe207 Main Chain Amide spectator Hydrogen Bond Donor

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
iron F3S 2509 A spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
N-H
O-H
O-H
O-H
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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