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Overview for MACiE Entry M0109

Version history

General Information

EC Number: 1.3.3.1 (A member of the Oxidoreductases, Acting on the CH-CH group of donors, With oxygen as acceptor)

Enzyme Name: dihydroorotate oxidase

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

  • Q02127 - Dihydroorotate dehydrogenase (quinone), mitochondrial

Representative PDB Code: 1d3g - HUMAN DIHYDROOROTATE DEHYDROGENASE COMPLEXED WITH BREQUINARANALOG (Resolution = 1.60 Å).

Catalytic CATH Codes:

Display structure information

Overall Reaction:

Image of ubiquinone

Image of (S)-dihydroorotate

right arrow

Image of ubiquinol

Image of orotate

ubiquinone
C00399
CHEBI:16389
(S)-dihydroorotate
C00337
CHEBI:30864
ubiquinol
C00390
CHEBI:17976
orotate
C00295
CHEBI:30839

View similar reactions


Stepwise Description of the Reaction

Step 1Ser215 deprotonates the C5 of dihydroorotate, eliminating a hydride ion, which is added to FMN.
Step 2The product dissociates from the active site, to be replaced by ubiquinone. The reduced FMN eliminates the hydride ion which is added to the ubiquinone, initiating double bond rearrangement and deprotonation of Ser215 to generate ubiquinol in an inferred return step.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Phe 149 A Side Chain
Ser 215 A Side Chain
Thr 218 A Side Chain
Lys 255 A Side Chain

Organic Cofactors for M0109

Type Identity Chain
FMN FMN 398 Overview

References

  1. S. Liu et al. (2000), Structure, 8, 25-33. Structures of human dihydroorotate dehydrogenase in complex with antiproliferative agents.
    Medline: 10673429

Homologue information for M0109 (1d3g)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0102 L-lactate dehydrogenase (cytochrome)
1.1.2.3
1fcb 3.20.20.70
0.55310.0237Compare
M0108 2,4-dienoyl-CoA reductase (NADPH)
1.3.1.34
1ps9 3.20.20.70
0.58330.0258Compare
M0111 glutamate synthase (ferredoxin)
1.4.7.1
1ofd 3.20.20.70
0.33540.1496Compare
M0114 trimethylamine dehydrogenase
1.5.8.2
2tmd 3.20.20.70
0.57140Compare
M0319 NADPH dehydrogenase
1.6.99.1
1oya 3.20.20.70
0.60860.09Compare
M0148 transaldolase
2.2.1.2
1onr 3.20.20.70
0.2230.3190Compare
M0008 nicotinate-nucleotide diphosphorylase (carboxylating)
2.4.2.19
1qpr 3.20.20.70
0.240.1442Compare
M0243 pyridoxine 5'-phosphate synthase
2.6.99.2
1ho1 3.20.20.70
0.31060.0408Compare
M0050 orotidine-5'-phosphate decarboxylase
4.1.1.23
1dbt 3.20.20.70
0.04540.1265Compare
M0252 indole-3-glycerol-phosphate synthase
4.1.1.48
1igs 3.20.20.70
0.31250.2548Compare
M0236 3-dehydro-L-gulonate-6-phosphate decarboxylase
4.1.1.85
1q6l 3.20.20.70
0.28570.1285Compare
M0052 fructose-bisphosphate aldolase (Class II)
4.1.2.13
1b57 3.20.20.70
0.44440Compare
M0222 fructose-bisphosphate aldolase (Class I)
4.1.2.13
2qut 3.20.20.70
0.19020.2329Compare
M0054 3-dehydroquinate dehydratase (type I)
4.2.1.10
1qfe 3.20.20.70
0.24210.1499Compare
M0230 porphobilinogen synthase
4.2.1.24
1gzg 3.20.20.70
0.34730.3195Compare
M0267 dihydrodipicolinate synthase
4.2.1.52
1dhp 3.20.20.70
0.44440.1499Compare
M0270 ribulose-phosphate 3-epimerase
5.1.3.1
1h1z 3.20.20.70
0.350.1351Compare
M0324 triosephosphate isomerase
5.3.1.1
1tph 3.20.20.70
0.280.2999Compare
M0223 pyruvate carboxylase
6.4.1.1
2qf7 3.20.20.70
0.39020.1125Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.20.20.70


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
dihydroorotate dehydrogenase activity (molecular function)
dihydroorotate oxidase activity (molecular function)
mitochondrion (cellular component)
mitochondrial inner membrane (cellular component)
pyrimidine nucleobase metabolic process (biological process)
'de novo' pyrimidine nucleobase biosynthetic process (biological process)
pyrimidine nucleotide biosynthetic process (biological process)
UMP biosynthetic process (biological process)
metabolic process (biological process)
membrane (cellular component)
integral component of membrane (cellular component)
oxidoreductase activity (molecular function)
positive regulation of apoptotic process (biological process)
pyrimidine nucleoside biosynthetic process (biological process)
nucleobase-containing small molecule metabolic process (biological process)
oxidation-reduction process (biological process)
regulation of mitochondrial fission (biological process)
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