Entry M0107    carbon-monoxide dehydrogenase (acceptor)

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Step 06

Glu763 deprotonates water, initiating a single electron transfer from the Mo(IV) centre, through two iron-sulfur clusters to FAD, and thence to an external electron acceptor.

This reaction is irreversible.

This reaction step has been inferred

GIF of Reaction Step M0107.stg06

Comment: Dobbek et al [1] suggest that Glu763 is acting as the base in deprotonating the Mo bound water ready for the next round of catalysis. This reaction may occur in more than a single step.


Proton Transfer
Electron Transfer

Mechanism Components

Overall Reactant Used
Electron Relay
Bond Formation
Bond Cleavage
Intermediate Formation
Cofactor Used
Cofactor Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu763 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Cys388 Side Chain spectator Metal Ligand
Electrostatic Stabiliser

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD3932 reactant One Electron Acceptor
One Electron Donor
Electron Relay

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
iron FES 3907 x reactant One Electron Donor
Electron Relay
One Electron Acceptor
iron FES 3908 x reactant One Electron Donor
Electron Relay
One Electron Acceptor
copper CUB 3921 x spectator Not Active
molybdenum CUB 3921 x reactant One Electron Donor
Substrate Binding
Increase Acidity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved

View similar reactions in MACiE.