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Entry M0106    1.2.4.1    pyruvate dehydrogenase (acetyl-transferring)

Previous Step

Step 08

His128 deprotonates water in an inferred return step.

This reaction step has been inferred

GIF of Reaction Step M0106.stg08



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu59 Side Chain spectator Hydrogen Bond Acceptor
His128 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Arg267C Side Chain spectator Not Active
His271C Side Chain spectator Not Active
Tyr281C Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Thiamine diphosphate TDP1370C spectator Metal Ligand
Hydrogen Bond Acceptor
Hydrogen Bond Donor

Metal Cofactors involved in Step 08

Metal Type Metal Identity Chain Activity Function
magnesium MG 1368 C spectator Cofactor Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
None
H
N
O

View similar reactions in MACiE.


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