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Entry M0106    1.2.4.1    pyruvate dehydrogenase (acetyl-transferring)

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Step 06

His271C deprotonates the hydroxyl in an elimination which reforms the carbanionic form of ThDP and produces the second product.

GIF of Reaction Step M0106.stg06



Mechanisms

Bimolecular Elimination

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu59 Side Chain spectator Hydrogen Bond Donor
His128 Side Chain spectator Hydrogen Bond Acceptor
Arg267C Side Chain spectator Not Active
His271C Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
Tyr281C Side Chain spectator Not Active

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Thiamine diphosphate TDP1370C reactant Metal Ligand
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Nucleofuge

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
magnesium MG 1368 C spectator Cofactor Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-C
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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