spacer

Entry M0106    1.2.4.1    pyruvate dehydrogenase (acetyl-transferring)

Previous Step     Next Step

Step 04

The covalently bound intermediate undergoes decarboxylation.

GIF of Reaction Step M0106.stg04



Mechanisms

Unimolecular Elimination by the Conjugate Base

Mechanism Components

Decarboxylation
Overall Product Formed
Bond Order Change
Bond Cleavage
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu59 Side Chain spectator Hydrogen Bond Donor
His128 Side Chain spectator Not Active
Arg267C Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His271C Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Tyr281C Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
Thiamine diphosphate TDP1370C reactant Metal Ligand
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Activator

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
magnesium MG 1368 C spectator Cofactor Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
C-C
The C-O bond changes from a single to double bond
C
O

View similar reactions in MACiE.


spacer
spacer