Overview for MACiE Entry M0105
EC Number: 18.104.22.168 (A member of the Oxidoreductases, Acting on the aldehyde or oxo group of donors, With oxygen as acceptor)
Enzyme Name: aldehyde oxidase
Biological Species: Desulfovibrio gigas (Bacteria)
Catalytic Chain UniprotKB Accession Codes:
- Q46509 - Aldehyde oxidoreductase
Representative PDB Code: 1vlb - STRUCTURE REFINEMENT OF THE ALDEHYDE OXIDOREDUCTASE FROMDESULFOVIBRIO GIGAS AT 1.28 A (Resolution = 1.28 Å).
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It is unknown whether this reaction is reversible or not.
Overall Comment: This molybdenum hydroxylase was shown to be part of an electron-transfer chain comprising four different soluble proteins from D. gigas with a total of 11 discrete redox centres.
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Stepwise Description of the Reaction
|Step 1||Glu869 deprotonates the water in the coordination sphere of Mo(VI), which initiates a nucleophilic attack on the aldehyde in a substitution reaction, which eliminates a hydride ion, which adds to the sulfur dianion which is also in the Mo(VI) coordination sphere, eventually reducing Mo(VI) to Mo(IV).|
|Step 2||Water deprotonates Glu869, which initiates a nucleophilic attack on the Mo(IV) centre, displacing the carboxylic acid product in a substitution reaction.|
|Step 3||A single electron is transferred from Mo(IV) to an acceptor via two iron-sulfur clusters.|
|Step 4||Water deprotonates the thiolate group part of the Mo(V) coordination sphere, causing the elimination of Glu869 from the Mo(V) coordination sphere, and transfer of the second electron from Mo(V) to the acceptor via two iron-sulfur clusters.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0105
- C. Kisker et al. (1997), Annu. Rev. Biochem., 66, 233-267. Molybdenum-cofactor-containing enzymes: structure and mechanism.
- B. A. S. Barata et al. (1993), Biochemistry, 32, 11559-11568. Aldehyde oxidoreductase activity in Desulfovibrio gigas: in vitro reconstitution of an electron-transfer chain from aldehydes to the production of molecular hydrogen.
- R. Huber et al. (1996), Proc. Natl Acad. Sci. USA, 93, 8846-8851. A structure-based catalytic mechanism for the xanthine oxidase family of molybdenum enzymes.
Homologue information for M0105 (1vlb)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
Entries with at least one Catalytic CATH code in common (different mechanisms):
|M0107 ||carbon-monoxide dehydrogenase (acceptor) |
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