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Entry M0104    1.1.5.2    quinoprotein glucose dehydrogenase

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Step 03

His144 deprotonates the C5 carbon of PQQ, forming the C4-oxyanion.

Rate Determining Step

GIF of Reaction Step M0104.stg03



Mechanisms

Proton Transfer
Assisted Keto-Enol Tautomerisation

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His144 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Hydrogen Bond Donor
Asp163 Side Chain spectator Hydrogen Bond Acceptor
Activator
Arg228 Side Chain spectator Hydrogen Bond Donor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
PQQ PQQ1 reactant Hydrogen Bond Donor
Proton Donor
Metal Ligand

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
calcium CA 1003 A spectator Cofactor Binding
Increase Acidity
Activator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-H
The C-C bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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