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Entry M0103    1.1.3.38    vanillyl-alcohol oxidase

Previous Step

Step 08

The pheonlate deprotonates Asp170.

GIF of Reaction Step M0103.stg08



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg504 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr503 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr108 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp170 Side Chain reactant Hydrogen Bond Donor
Proton Donor
His422 Side Chain spectator Covalently Attached

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD600 spectator Covalently Attached
Hydrogen Bond Acceptor
Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
None
H
O

View similar reactions in MACiE.


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