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Entry M0103    1.1.3.38    vanillyl-alcohol oxidase

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Step 04

The dioxygen molecule undergoes a homolytic reaction in which it colligates to FAD, with concomitant deprotonation of Asp170.

GIF of Reaction Step M0103.stg04


Comment: It is assumed the Asp170 acts as the acid in this step.



Mechanisms

Colligation
Proton Transfer

Mechanism Components

Bond Formation
Enzyme-Substrate Bond Formation
Bond Cleavage
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg504 Side Chain spectator Hydrogen Bond Donor
Tyr503 Side Chain spectator Hydrogen Bond Donor
Tyr108 Side Chain spectator Hydrogen Bond Donor
Asp170 Side Chain reactant Hydrogen Bond Donor
Proton Donor
His422 Side Chain spectator Covalently Attached
Activator

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD600 reactant Covalently Attached
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Radical Combinant

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
O-H
O-H
None
C
H
O

View similar reactions in MACiE.


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