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Entry M0103    1.1.3.38    vanillyl-alcohol oxidase

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Step 03

The FAD undergoes double bond rearrangement which causes a single electron to be transferred to a dioxygen molecule.

GIF of Reaction Step M0103.stg03



Mechanisms

Electron Transfer
Radical Formation

Mechanism Components

Overall Reactant Used
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg504 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr503 Side Chain spectator Hydrogen Bond Donor
Tyr108 Side Chain spectator Hydrogen Bond Donor
Asp170 Side Chain spectator Hydrogen Bond Donor
His422 Side Chain spectator Covalently Attached
Activator

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD600 reactant Covalently Attached
Hydrogen Bond Acceptor
Hydrogen Bond Donor
One Electron Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
The C-N bond changes from a single to double bond
The C-C bond changes from a double to single bond
The O-O bond changes from a double to single bond
C
N
O

View similar reactions in MACiE.


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