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Entry M0102    1.1.2.3    L-lactate dehydrogenase (cytochrome)

Next Step

Step 01

His373 deprotonates the alcohol of the lactate which eliminates a hydride ion that is added to FAD.

It is unknown whether this reaction is reversible or not.

GIF of Reaction Step M0102.stg01



Mechanisms

Bimolecular Elimination
Hydride Transfer
Aromatic Bimolecular Nucleophilic Addition

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Bond Order Change
Cofactor Used
Intermediate Formation
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr254 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp282 Side Chain spectator Hydrogen Bond Acceptor
His373 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
Hydrogen Bond Donor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FMN FMN570 reactant Hydrogen Bond Acceptor
Hydride Acceptor

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
iron HEM 560 A spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
N-H
C-H
O-H
The C-C bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-N bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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