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Overview for MACiE Entry M0102

Version history

General Information

EC Number: 1.1.2.3 (A member of the Oxidoreductases, Acting on the CH-OH group of donors, With a cytochrome as acceptor)

Enzyme Name: L-lactate dehydrogenase (cytochrome)

Biological Species: Saccharomyces cerevisiae (Baker's yeast)

Catalytic Chain UniprotKB Accession Codes:

  • P00175 - Cytochrome b2, mitochondrial

Representative PDB Code: 1fcb - MOLECULAR STRUCTURE OF FLAVOCYTOCHROME B2 AT 2.4 ANGSTROMS RESOLUTION (Resolution = 2.40 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of (S)-lactate

Image of ferricytochrome c

right arrow

Image of proton

Image of ferrocytochrome c

Image of pyruvate

(S)-lactate
C00186
CHEBI:16651
2 ferricytochrome c
C00125
CHEBI:16448
2 proton
C00080
CHEBI:24636
2 ferrocytochrome c
C00126
CHEBI:16707
pyruvate
C00022
CHEBI:15361

This reaction is irreversible.

Overall Comment: An alternative mechanism which proceeds via a carbanion has also been proposed. However Mowat et al. [1] firmly support the mechanism shown here, i.e. the hydride transfer mechanism.


View similar reactions


Stepwise Description of the Reaction

Step 1His373 deprotonates the alcohol of the lactate which eliminates a hydride ion that is added to FAD.
Step 2A single electron is transferred from the FAD to the haem cofactor.
Step 3The haem cofactor transfers the electron to ferricytochrome c.
Step 4Water deprotonates the FAD, initiating the second single electron transfer from FAD to the haem cofactor.
Step 5The haem cofactor transfers the electron to ferricytochrome c.
Step 6Water deprotonates His373 in an inferred return step.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Tyr 254 A Side Chain
Asp 282 A Side Chain
His 373 A Side Chain

Organic Cofactors for M0102

Type Identity Chain
FMN FMN 570 A Overview

Metal Cofactors for M0102

Type Het group Number Chain
iron HEM 560 A Overview

References

  1. C. G. Mowat et al. (2004), Biochemistry, 43, 9519-9526. Altered substrate specificity in flavocytochrome b2: structural insights into the mechanism of L-lactate dehydrogenation.
    Medline: 15260495
  2. M. Gondry et al. (2001), Eur. J. Biochem., 268, 4918-4927. The catalytic role of tyrosine 254 in flavocytochrome b2 (L-lactate dehydrogenase from baker's yeast). Comparison between the Y254F and Y254L mutant proteins.
    Medline: 11559361

Homologue information for M0102 (1fcb)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0108 2,4-dienoyl-CoA reductase (NADPH)
1.3.1.34
1ps9 3.20.20.70
0.34250.3162Compare
M0109 dihydroorotate oxidase
1.3.3.1
1d3g 3.20.20.70
0.55310.0237Compare
M0111 glutamate synthase (ferredoxin)
1.4.7.1
1ofd 3.20.20.70
0.27380Compare
M0114 trimethylamine dehydrogenase
1.5.8.2
2tmd 3.20.20.70
0.55260.45Compare
M0319 NADPH dehydrogenase
1.6.99.1
1oya 3.20.20.70
0.52270.2414Compare
M0148 transaldolase
2.2.1.2
1onr 3.20.20.70
0.10260.1622Compare
M0008 nicotinate-nucleotide diphosphorylase (carboxylating)
2.4.2.19
1qpr 3.20.20.70
0.12240.1499Compare
M0243 pyridoxine 5'-phosphate synthase
2.6.99.2
1ho1 3.20.20.70
0.17410.1496Compare
M0050 orotidine-5'-phosphate decarboxylase
4.1.1.23
1dbt 3.20.20.70
0.02630.3157Compare
M0252 indole-3-glycerol-phosphate synthase
4.1.1.48
1igs 3.20.20.70
0.14920.0145Compare
M0236 3-dehydro-L-gulonate-6-phosphate decarboxylase
4.1.1.85
1q6l 3.20.20.70
0.23680.3738Compare
M0052 fructose-bisphosphate aldolase (Class II)
4.1.2.13
1b57 3.20.20.70
0.14510.18Compare
M0222 fructose-bisphosphate aldolase (Class I)
4.1.2.13
2qut 3.20.20.70
0.10510.1575Compare
M0054 3-dehydroquinate dehydratase (type I)
4.2.1.10
1qfe 3.20.20.70
0.21960.1913Compare
M0230 porphobilinogen synthase
4.2.1.24
1gzg 3.20.20.70
0.22050.013Compare
M0267 dihydrodipicolinate synthase
4.2.1.52
1dhp 3.20.20.70
0.49230.1967Compare
M0270 ribulose-phosphate 3-epimerase
5.1.3.1
1h1z 3.20.20.70
0.1750.2999Compare
M0324 triosephosphate isomerase
5.3.1.1
1tph 3.20.20.70
0.05550.1499Compare
M0223 pyruvate carboxylase
6.4.1.1
2qf7 3.20.20.70
0.25880.2999Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.20.20.70


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

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catalytic activity (molecular function)
metabolic process (biological process)
FMN binding (molecular function)
oxidoreductase activity (molecular function)
heme binding (molecular function)
oxidation-reduction process (biological process)
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