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Entry M0101    3.6.1.29    bis(5'-adenosyl)-triphosphatase

Previous Step

Step 02

ADP deprotonates water, which attacks the phosphate of the covalently bound AMP, eliminating His96 and proceeding via a pentavalent transition state.

GIF of Reaction Step M0101.stg02


Comment: We assume that ADP acts as the general acid.



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Enzyme-Substrate Bond Cleavage
Intermediate Collapse
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated
Hydrolysis

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Gln83 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His96 Side Chain reactant Nucleofuge
His98 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
P-O
O-H
P-N
None
H
N
O
P

View similar reactions in MACiE.


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