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Entry M0101    3.6.1.29    bis(5'-adenosyl)-triphosphatase

Next Step

Step 01

His96 attacks the alpha phosphate of the substrate in a nucleophilic attack which proceeds via a pentavalent transition state and results in the elimination of ADP.

GIF of Reaction Step M0101.stg01


Comment: Reaction proceeds through a trigonal bipimrimidal pentavalent transition state, which inverts the position of the alpha phosphate.



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Enzyme-Substrate Bond Formation
Bond Cleavage
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Gln83 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His96 Side Chain reactant Nucleophile
His98 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-N
P-O
None
N
O
P

View similar reactions in MACiE.


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