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Overview for MACiE Entry M0097

Version history

General Information

EC Number: 3.5.4.5 (A member of the Hydrolases, Acting on carbon-nitrogen bonds, other than peptide bonds, In cyclic amidines)

Enzyme Name: cytidine deaminase

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

Representative PDB Code: 1ctt - TRANSITION-STATE SELECTIVITY FOR A SINGLE OH GROUP DURING CATALYSIS BYCYTIDINE DEAMINASE (Resolution = 2.20 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of cytidine

Image of water

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Image of uridine

Image of ammonia

cytidine
C00475
CHEBI:17562
water
C00001
CHEBI:15377
uridine
C00299
CHEBI:16704
ammonia
C00014
CHEBI:16134

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Stepwise Description of the Reaction

Step 1Glu104 deprotonates water.
Step 2Activated water initiates a nucleophilic attack on the substrate in an addition reaction with concomitant deprotonation of Glu104.
Step 3Glu104 deprotonates the added hydroxyl group.
Step 4The oxyanion collapses, eliminating ammonia with concomitant deprotonation of Glu104.

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Catalytic Residues Involved

Type Number Chain Location of Function
Glu 104 A Side Chain

Metal Cofactors for M0097

Type Het group Number Chain
zinc ZN 296 x Overview

References

  1. S. Xiang et al. (1997), Biochemistry, 36, 4768-4774. The structure of the cytidine deaminase-product complex provides evidence for efficient proton transfer and ground-state destabilization.
    Medline: 9125497

Homologue information for M0097 (1ctt)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
cytidine deaminase activity (molecular function)
zinc ion binding (molecular function)
cytidine deamination (biological process)
hydrolase activity (molecular function)
cytidine metabolic process (biological process)
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